KCTBASE

방사광 소각 X-선 산란을 이용한 쥐 궁둥신경의 주사바늘에 의한 신경손상 유무에 관한 연구
Peripheral nerve injury caused by needle impalement: Synchrotron small-angle X-ray scattering study in ex-vivo rat sciatic nerve

Anesthesia and Pain Medicine 2014년 9권 4호 p.268 ~ p.273

김봉일(Kim Bong-Il) - 대구가톨릭대학교 의과대학 마취통증의학교실
송석영(Song Seok-Young) - 대구가톨릭대학교 의과대학 마취통증의학교실
류태하(Ryu Tae-Ha) - 대구가톨릭대학교 의과대학 마취통증의학교실
최영환(Choi Young-Hwan) - 대구가톨릭대학교 의과대학 마취통증의학교실
정진용(Jung Jin-Yong) - 대구가톨릭대학교 의과대학 마취통증의학교실
신태주(Shin Tae-Joo) - 포항가속기연구소

Abstract

Background: Direct puncture by a needle is a risk factor for nerve damage. This study was designed to demonstrate nerve damage caused by a needle using the synchrotron small-angle X-ray scattering (SAXS) technique.

Methods: A 15 mm section of rat (Male Spargue-Dawley, about 250 grams) sciatic nerves were involved in this study. The nerve specimen for the experiment (N = 5) was punctured 5 times by a needle (25 G, 100 beveled) under general anesthesia with enflurane. The needle was placed perpendicular to the nerve and the needle bevel was placed parallel to the nerve. The SAXS patterns of the punctured nerves, extracted about 15 min prior to the experiment, were acquired after 1 week. The SAXS patterns of a normal sciatic nerve (N = 5), extracted about 15 min prior to the experiment, were measured in order to provide a comparison. Experiments were carried out at 4C1 beamline at Pohang Accelerator Laboratory in Korea. Incoming X-rays were monochromatized at 11 keV using a double multilayer (WB4C) monochromator; the beam size was around 0.5 (V) × 0.8 (H) mm2. The exposure time was 60 sec, and 8 to 12 images were acquired per sample with a 0.5 mm interval.

Results: In the punctured group, the periodic peaks of myelin sheath and collagen fiber were not changed. However, the periodic peaks of interfibrillar distance of collagen were greatly changed.

Conclusions: Direct needle-nerve impalement did not cause damages in myelin sheath and collagen fibers when the needle was placed perpendicular and the needle bevel paralleled to the nerve fiber. This result can imply that the needle slipped into the interfibrillar packing of collagen fibrils. (Anesth Pain Med 2014; 9: 268-273)

키워드

Needle, Scattering, Sciatic nerve, Small angle

KMID : 1155520140090040268

원문 및 링크아웃 정보

등재저널 정보

주제코드

주제명(Target field)

연구대상(Population)

연구참여(Sample size)

대상성별(Gender)

질병특성(Condition Category)

연구환경(Setting)

연구설계(Study Design)

연구기간(Period)

중재방법(Intervention Type)

중재명칭(Intervention Name)

키워드(Keyword)

유효성결과(Recomendation)

연구비지원(Fund Source)

근거수준평가(Evidence Hierarchy)

출판년도(Year)

참여저자수(Authors)

대표저자

DOI

KCD코드

ICD 03

건강보험코드

방사광 소각 X-선 산란을 이용한 쥐 궁둥신경의 주사바늘에 의한 신경손상 유무에 관한 연구 Peripheral nerve injury caused by needle impalement: Synchrotron small-angle X-ray scattering study in ex-vivo rat sciatic nerve

Abstract

키워드

방사광 소각 X-선 산란을 이용한 쥐 궁둥신경의 주사바늘에 의한 신경손상 유무에 관한 연구
Peripheral nerve injury caused by needle impalement: Synchrotron small-angle X-ray scattering study in ex-vivo rat sciatic nerve