Polymorphisms of DNA Repair Gene XRCC1 and Radiation Sensitivity

Journal of Lung Cancer 2004년 3권 1호 p.31 ~ p.37

전미선(Chun Mi-Son) - 아주대학교 의과대학
최은경(Choi Eun-Kyung) - 울산대학교 의과대학 서울아산병원 방사선종양학과
박헌주(Park Heon Joo) - 인하대학교 의과대학 미생물학교실
홍윤철(Hong Yun-Chul) - 인하대학교 의과대학 예방의학교실
윤상민(Yoon Sang-Min) - 울산대학교 의과대학 서울아산병원 방사선종양학과
김영석(Kim Young-Seok) - 울산대학교 의과대학 서울아산병원 방사선종양학과
김종훈(Kim Jong-Hoon) - 울산대학교 의과대학 서울아산병원 방사선종양학과
안승도(Ahn Seung-Do) - 울산대학교 의과대학 서울아산병원 방사선종양학과
이상욱(Lee Sang-Wook) - 울산대학교 의과대학 서울아산병원 방사선종양학과
신성수(Shin Seong-Soo) - 울산대학교 의과대학 서울아산병원 방사선종양학과
박찬일(Park Charn-Il) - 서울대학교 의과대학 방사선종양학교실

Abstract

Purpose: The goal of this study was to find lung cancer-related single nucleotide polymorphisms (SNP) and define their association with clinical results.

Material and Methods: One hundred and thirty-six non-small cell lung cancer patients, who received radiotherapy at the Asan Medical Center, were recruited between August 2002 and September 2003. Demographic and clinical informations were obtained from a self-administered questionnaire and from the subject’s medical records, respectively. Blood samples were collected from all study subjects at the time of enrollment. Genomic DNA was extracted from peripheral blood lymphocytes using a QIAamp DNA Blood Mini Kit. TaqMan assay, denaturing HPLC and single base pair primer extension assay using SNaPshot kits were employed as the SNP screening techniques. The candidate SNP for screening was XRCC1-R399Q.

Results: Patients carrying the 399Gln variant allele had a significantly longer progression-free survival than those with the 399Arg homozygote in tumor stages I-IIIa (p=0.005). In the Cox-proportional hazards model, the XRCC1 codon 399 polymorphism was a statistically significant predictor for progression-free survival in tumor stages I-IIIa (p=0.03).
Conclusion: The use of molecular predictors of the progression-free survival in non-small cell lung cancer patients, particularly at stages I-IIIa, may provide important criteria for prognosis of the patients undergoing radiotherapy. However, there is still a need for further study to establish the role of these polymorphisms as useful predictors.

키워드

Single nucleotide polymorphism (SNP), Lung cancer, Radiotherapy
원문 및 링크아웃 정보
등재저널 정보
주제코드
주제명(Target field)
연구대상(Population)
연구참여(Sample size)
대상성별(Gender)
질병특성(Condition Category)
연구환경(Setting)
연구설계(Study Design)
연구기간(Period)
중재방법(Intervention Type)
중재명칭(Intervention Name)
키워드(Keyword)
유효성결과(Recomendation)
Patients carrying the 399Gln variant allele had a significantly longer progression-free survival than those with the 399Arg homozygote in tumor stages I-IIIa (p=0.005); The use of molecular predictors of the progression-free survival in non-small cell lung cancer patients, particularly at stages I-IIIa, may provide important criteria for prognosis of the patients undergoing radiotherapy.
연구비지원(Fund Source)
근거수준평가(Evidence Hierarchy)
출판년도(Year)
참여저자수(Authors)
대표저자
DOI
KCD코드
ICD 03
건강보험코드