Glutathione Protects Brain Endothelial Cells from Hydrogen Peroxide-Induced Oxidative Stress by Increasing Nrf2 Expression
Experimental Neurobiology 2014³â 23±Ç 1È£ p.93 ~ p.103
¼ÛÁÖÇö(Song Ju-Hyun) - Yonsei University College of Medicine Department of Anatomy
(Kang So-Mang) - Yonsei University College of Medicine Department of Anatomy
ÀÌ¿øÅÃ(Lee Won-Taek) - Yonsei University College of Medicine Department of Anatomy
¹Ú°æ¾Æ(Park Kyung-Ah) - Yonsei University College of Medicine Department of Anatomy
ÀÌ°æ¹Î(Lee Kyoung-Min) - Seoul National University College of Medicine Department of Neurology
ÀÌÁ¾Àº(Lee Jong-Eun) - Yonsei University College of Medicine Department of Anatomy
Abstract
Glutathione (GSH) protects cells against oxidative stress by playing an antioxidant role. Protecting brain endothelial cells under oxidative stress is key to treating cerebrovascular diseases and neurodegenerative diseases including Alzheimer¡¯s disease and Huntington¡¯s disease. In present study, we investigated the protective effect of GSH on brain endothelial cells against hydrogen peroxide (H2O2). We showed that GSH attenuates H2O2-induced production of nitric oxide (NO), reactive oxygen species (ROS), and 8-Oxo-2¡¯-deoxyguanosine (8-OHdG), an oxidized form of deoxiguanosine. GSH also prevents H2O2-induced reduction of tight junction proteins. Finally, GSH increases the level of nuclear factor erythroid 2-related factor 2 (Nrf2) and activates Nrf2-mediated signaling pathways. Thus, GSH is a promising target to protect brain endothelial cells in conditions of brain injury and disease.
Å°¿öµå
glutathione (GSH), murine brain endothelial cells (bEnd.3 cells), apoptosis, hydrogen peroxide (H2O2), Reactive oxygen species (ROS), nuclear factor erythroid 2-related factor 2 (Nrf2)
KMID :
0892920140230010093
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