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A Study of Efflux Pump Genes in Mycobacterium tuberculosis Clinical Isolates
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Á¶¼±¿µ(Cho Sun-Young) - °æÈñ´ëÇб³ ÀÇ°ú´ëÇÐ Áø´Ü°Ë»çÀÇÇб³½Ç
³²À¯¼±(Nam You-Sun) - °æÈñ´ëÇб³ ´ëÇпø
¾çÇü¼®(Yang Hyung-Seok) - °æÈñ´ëÇб³ ÀÇ°ú´ëÇÐ Áø´Ü°Ë»çÀÇÇб³½Ç
¼ÒÀ±¼ö(Soh Yun-Soo) - °æÈñ´ëÇб³ ÀÇ°ú´ëÇÐ Áø´Ü°Ë»çÀÇÇб³½Ç
±è¹ÎÁø(Kim MIn-Jin) - ¾¾Á¨ÀÇ·áÀç´Ü
ÀÌÈñÁÖ(Lee Hee-Joo) - °æÈñ´ëÇб³ ÀÇ°ú´ëÇÐ Áø´Ü°Ë»çÀÇÇб³½Ç
Abstract
Efflux pump systemÀº ¾àÁ¦ ³»¼º °áÇÙ±ÕÀÇ Áß¿äÇÑ ±âÀü Áß Çϳª·Î Á¦¾ÈµÇ°í ÀÖ´Ù. ÀÌ ¿¬±¸´Â efflux pump systemÀÇ 5°¡Áö À¯ÀüÀÚ¿¡ ´ëÇÑ ºÐ¼®À» ÅëÇØ, Çѱ¹ ȯÀÚµé·ÎºÎÅÍ ºÐ¸®µÈ °áÇÙ±ÕÀÇ ÀûÀýÇÑ Ç¥ÀûÀ» ¼±ÅÃÇÏ°í Á¦¾ÈÇϱâ À§ÇØ ½ÃÇàµÇ¾ú´Ù.ÀÌ ¿¬±¸¿¡¼´Â ¾ç¼º µ¿Á¤°á°ú¸¦ º¸ÀÌ´Â 35 °Ëü°¡ ºÐ¼®µÇ¾ú´Ù. mmpL7, efpA, mmr, p55, ±×¸®°í tap-like gene µî 5Á¾·ùÀÇefflux gene¿¡ ´ëÇØ PCRÀ» ½ÃÇàÇÏ¿© 35°³ÀÇ ºÐ¸®µÈ °áÇÙ ±ÕÁÖ¸¦ ºÐÀÚÀû ¹æ¹ýÀ¸·Î ºÐ¼®ÇÏ¿´´Ù. ±× °á°ú ¿ÀÁ÷ ÇϳªÀÇ ºÐ¸®±ÕÁÖ¿¡¼ 5Á¾·ùÀÇ efflux pump gene PCR¿¡ ´ëÇØ À½¼º °á°ú¸¦ º¸¿´´Ù. ³ª¸ÓÁö 34°³ÀÇ ºÐ¸® ±ÕÁÖ¿¡¼´Â 5Á¾·ùÀÇ effluxpump gene¿¡ ´ëÇØ µ¿½Ã ¾ç¼º °á°ú°¡ ³ª¿Ô´Ù. ¾ÕÀ¸·Î ÀÌ À¯ÀüÀڵ鿡 ´ëÇÑ PCR Á¤·® °Ë»ç¸¦ ÅëÇØ À¯ÀüÀÚ ¹ßÇö Á¶»ç°¡ÁøÇàµÇ¾î¾ß ÇÒ °ÍÀÌ´Ù.
The efflux pump system has been suggested as an important mechanism in the drug resistance of Mycobacterium tuberculosis (MTB). In this study, molecular analysis of five genes in the efflux pump system of MTB isolates from Korean patients was performed in order to identify appropriate molecular targets. In this study, 35 culture-positive specimens were included. PCR was performed for five efflux genes, mmpL7, efpA, mmr, p55 and tap-like gene. In the 35 clinical isolates, molecular analysis of five kinds of efflux pump genes was performed. Only one clinical isolate showed negative PCR results for all five efflux pump genes. All the rest 34 isolates presented concurrent positive results for the five efflux pump genes. In the near future, gene expressi on study with quantitative PCR should be performed using these genes.
Å°¿öµå
Multidrug efflux pump genes, Mycobacterium tuberculosis, Drug resistance
KMID :
0829320140170020065
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