Knockdown of 14-3-3¥æ enhances radiosensitivity and radio-induced apoptosis in CD133+ liver cancer stem cells

Experimental & Molecular Medicine 2014³â 46±Ç 2È£ p.5 ~ p.0

ÀÌ¿µ±â(Lee Young-Ki) - Catholic University Liver Research Center and WHO Collaborating Center of Viral hepatitis
Çã¿øÈñ(Hur Won-Hee) - Catholic University Liver Research Center and WHO Collaborating Center of Viral hepatitis
À̼º¿ø(Lee Sung-Won) - Catholic University Liver Research Center and WHO Collaborating Center of Viral hepatitis
È«¼º¿ì(Hong Sung-Woo) - Catholic University Liver Research Center and WHO Collaborating Center of Viral hepatitis
±è¼º¿ì(Kim Sung-Woo) - Catholic University Liver Research Center and WHO Collaborating Center of Viral hepatitis
ÃÖÁ¤Àº(Choi Jung-Eun) - Catholic University Liver Research Center and WHO Collaborating Center of Viral hepatitis
À±½Â±Ô(Yoon Seung-Kew) - Catholic University Liver Research Center and WHO Collaborating Center of Viral hepatitis

Abstract

14-3-3¥æ is related to many cancer survival cellular processes. In a previous study, we showed that silencing 14-3-3¥æ decreases the resistance of hepatocellular carcinoma (HCC) to chemotherapy. In this study, we investigated whether silencing 14-3-3¥æ affects the radioresistance of cancer stem-like cells (CSCs) in HCC. Knockdown of 14-3-3¥æ decreased cell viability and the number of spheres by reducing radioresistance in CSCs after ¥ã-irradiation (IR). Furthermore, the levels of pro-apoptotic proteins were upregulated in CSCs via silencing 14-3-3¥æ after IR. These results suggest that 14-3-3¥æ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver CSCs.

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14-3-3¥æ, apoptosis, cancer stem-like cell, hepatocellular carcinoma, radioresistance
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14-3-3¥æ knockdown enhances radio-induced apoptosis by reducing radioresistance in liver cancer stem-like cells
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