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Applied the ¡°Type and Screen¡± Method Based on Antibody Screening Test Including Dia Cells and Immediate Spin Crossmatch to All Patients Who Received Transfusion for Five Years

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±èÇý¸²(Kim Hye-Rim) - ºÎ»ê´ëÇб³ ÀÇ°ú´ëÇÐ Áø´Ü°Ë»çÀÇÇб³½Ç
±èÇüȸ(Kim Hyung-Hoi) - ºÎ»ê´ëÇб³ ÀÇ°ú´ëÇÐ Áø´Ü°Ë»çÀÇÇб³½Ç

Abstract

Background: To prevent a hemolytic transfusion reaction caused by an unexpected antibody, a crossmatch following ABO/Rh typing and antibody screening test is performed before a red cell transfusion. The conventional crossmatch method is usually performed using the room temperature saline method, 37oC albumin method, and anti-globulin method. With growth and development of techniques for laboratory and records management, demand for reduction of work load is on the rise, therefore, use of the ¡°type and screen¡± method and Immediate spin crossmatch as a pretransfusion test is recommended.

Methods: A retrospective analysis was performed using records from the Pusan National University Hospital blood bank from 2008 to 2012. Room temperature saline method omitting the anti-globulin crossmatch has been used in order to issue a compatible red cell product, when antibody screening performed using a cell panel (DiaCell I-II-Dia) in all patients where a request for a red cell transfusion was sent to the blood bank is negative, without previously identified unexpected antibodies.

Results: In total, 107,569 units of PRBCs were requested, 100,978 units (93.88%) were issued only immediate-spin crossmatch following type and screen method, and exclusion criteria were indicated in 6.12% of the total. There was no single case where the antibody screen was negative with guarantee of compatibility and hemolytic transfusion reaction occurred.

Conclusion: The ¡°Type and Screen¡± method based on antibody screening test including Dia cells and Immediate spin crossmatch application can contribute to safe, efficient transfusion service in the blood bank.

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Type and screen method, Antibody screening, Transfusion, Cross-matching
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The ¡°Type and Screen¡± method based on antibody screening test including Dia cells and Immediate spin crossmatch application can contribute to safe, efficient transfusion service in the blood bank.
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DOI
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ICD 03
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