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인간 섬유아세포에 대한 마이토마이신 C와 피르페니돈의 독성과 항섬유화효과
Cytotoxicities and Anti-Fibrotic Effects of Pirfenidone and Mitomycin C on Human Fibroblasts

대한안과학회지 2014년 55권 7호 p.1077 ~ p.1083

박경수(Park Kyoung-Soo) - 실로암 안과병원
홍사민(Hong Sa-Min) - 연세대학교 의과대학 안과학교실
이주카요코(Iizuka Yoko) - 연세대학교 의과대학 안과학교실
김찬윤(Kim Chan-Yun) - 연세대학교 의과대학 안과학교실
성공지(Seong Gong-Je) - 연세대학교 의과대학 안과학교실

Abstract

Purpose: The cytotoxicities and anti-fibrotic effects of mitomycin C and pirfenidone on human dermal fibroblast were evaluated.

Methods: Initially, 24-hour cell cultures were exposed to transforming growth factor (TGF)-β1, different concentrations of mitomycin C, and pirfenidone solutions in order to evaluate cytotoxicity. Expressions of fibronectin, collagen type 1, α smooth muscle, and β-actin were evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) and western blot in mitomycin C solutions at concentrations of 4 μg/mL and 20 μg/mL, and in pirfenidone solutions at 250 μg/mL and 500 μg/mL.

Results: In comparison to cell cultures exposed to TGF-β1 solutions, cytotoxicities were increased in solutions of mitomycin C at 4 μg/mL, 20 μg/mL, 40 μg/mL and pirfenidone at 500 μg/mL, 750 μg/mL, 1,000 μg/mL (p < 0.05, Mann Whitney U-test). The results of real-time RT-PCR show that expressions of fibronectin, collagen type 1, and α smooth muscle were significantly more decreased in all concentrations of mitomycin C and pirfenidone compared to those in TGF-β1 solution. In western blot analysis, expressions of fibronectin and α smooth muscle were decreased in all concentrations of mitomycin C and pirfenidone compared to TGF-β1 solution.

Conclusions: Both drugs have cytotoxicities and anti-fibrotic effects, but pirfenidone was found to have less cytotoxicity and mitomycin C was found to have more anti-fibrotic effects when compared to each other.

키워드

Anti-fibrotic effect, Cytotoxicity, Mitomycin C, Pirfenidone

KMID : 0360220140550071077

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ICD 03

건강보험코드

인간 섬유아세포에 대한 마이토마이신 C와 피르페니돈의 독성과 항섬유화효과 Cytotoxicities and Anti-Fibrotic Effects of Pirfenidone and Mitomycin C on Human Fibroblasts

Abstract

키워드

인간 섬유아세포에 대한 마이토마이신 C와 피르페니돈의 독성과 항섬유화효과
Cytotoxicities and Anti-Fibrotic Effects of Pirfenidone and Mitomycin C on Human Fibroblasts