A comparison of antioxidant activity of Korean White and Red Ginsengs on H2O2-induced oxidative stress in HepG2 hepatoma cells

Journal of Ginseng Research 2013년 37권 4호 p.442 ~ p.450

손상현(Sohn Sang-Hyun) - Rural Development Administration National Institute of Horticultural & Herbal Science Department of Medicinal Crop Research
김시관(Kim Si-Kwan) - Konkuk University College of Biomedical & Health Science Department of Biomedical Chemistry
김영옥(Kim Young-Ock) - Rural Development Administration National Institute of Horticultural & Herbal Science Department of Medicinal Crop Research
김형돈(Kim Hyung-Don) - Rural Development Administration National Institute of Horticultural & Herbal Science Department of Medicinal Crop Research
신유수(Shin Yu-Su) - Rural Development Administration National Institute of Horticultural & Herbal Science Department of Medicinal Crop Research
양승옥(Yang Seung-Ok) - Rural Development Administration National Institute of Horticultural & Herbal Science Department of Medicinal Crop Research
김승유(Kim Seung-Yu) - Rural Development Administration National Institute of Horticultural & Herbal Science Department of Medicinal Crop Research
이상원(Lee Sang-Won) - Rural Development Administration National Institute of Horticultural & Herbal Science Department of Medicinal Crop Research

Abstract

The aim of this study was to determine and compare the preventive effect of Korean White Ginseng and Red Ginseng on
oxidative stress in H2O2-treated HepG2 cells. The roots of ginseng were extracted with 70% methanol and partitioned with butanol to obtain saponin fractions, which have been known as bioactive constituents of ginseng. 2′,7′-Dichlorofluorescein diacetate (DCF-DA) assay and malondialdehyde (MDA) content were measured for evaluating intracellular reactive oxygen species (ROS) generation. Also, mRNA expressions and activities of antioxidant enzymes were analyzed to determine the antioxidant activity of saponin or non-saponin fractions of ginsengs. According to DCF-DA assay, H2O2-induced MDA release and ROS generation were significantly reduced by treatment with saponin fractions of white and red ginseng roots. Also, saponin fractions increased effectively intracellular antioxidant enzyme activities including catalase, glutathione peroxidase and superoxide dismutase in H2O2-treated HepG2 hepatoma cells. In general, red ginseng was more effective than white ginseng for reducing oxidative stress. These results indicate that administration of red ginseng may certainly contribute relatively stronger than white ginseng to prevent from damaging liver function by oxidative stress.

키워드

Panax ginseng, Oxidative stress, HepG2 hepatoma cell, Antioxidant enzyme,
원문 및 링크아웃 정보
등재저널 정보
학술진흥재단(KCI) 
주제코드
주제명(Target field)
연구대상(Population)
연구참여(Sample size)
대상성별(Gender)
질병특성(Condition Category)
연구환경(Setting)
연구설계(Study Design)
연구기간(Period)
중재방법(Intervention Type)
중재명칭(Intervention Name)
키워드(Keyword)
유효성결과(Recomendation)
Administrating red ginseng may certainly contribute relatively stronger than white ginseng to prevent from damaging liver function by oxidative stress.
연구비지원(Fund Source)
근거수준평가(Evidence Hierarchy)
출판년도(Year)
참여저자수(Authors)
대표저자
KCD코드
ICD 03
건강보험코드