Sohn, Yujin; Jeong, Su Jin; Chung, Won Suk; Hyun, Jong Hoon; Baek, Yae Jee; Cho, Yunsuk; Kim, Jung Ho; Ahn, Jin Young; Choi, Jun Yong; Yeom, Joon-Sup
Journal of clinical medicine
2020Sep ; 9 ( 9 ) :.
PMID : 32927798
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Sohn, Yujin - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro Seodaemun-gu, Seoul 03722, Korea.
Jeong, Su Jin - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
Chung, Won Suk - College of Social Science, Yonsei University Graduate School, Seoul 03722, Korea.
Hyun, Jong Hoon - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
Baek, Yae Jee - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
Cho, Yunsuk - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
Kim, Jung Ho - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
Ahn, Jin Young - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
Choi, Jun Yong - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
Yeom, Joon-Sup - Division of Infectious Disease, Department of Internal Medicine, Severance Hospital,
ABSTRACT
BACKGROUND: The coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a major global public health issue. SARS-CoV-2 infection is confirmed by the detection of viral RNA using reverse transcription polymerase chain reaction (RT-PCR). Prolonged viral shedding has been reported in patients with SARS-CoV-2 infection, but the presence of viral RNA does not always correlate with infectivity. Therefore, the present study aimed to confirm the presence of viable virus in asymptomatic or mildly symptomatic patients in the later phase of the disease, more than two weeks after diagnosis. METHOD: Asymptomatic or mildly symptomatic COVID-19 patients who had been diagnosed with the disease at least two weeks previously and admitted to a community treatment center (CTC) from 15 March to 10 April 2020 were enrolled in this study. Nasopharyngeal and salivary swab specimens were collected from each patient. Using these specimens, RT-PCR assay and viral culture were performed. RESULT: In total, 48 patients were enrolled in this study. There were no significant differences in baseline characteristics between the asymptomatic and mildly symptomatic patient groups. RT-PCR assay and viral culture of SARS-CoV-2 were performed using nasopharyngeal and salivary swabs. The results of RT-PCR performed using salivary swab specimens, in terms of cycle threshold (Ct) values, were similar to those of RT-PCR using nasopharyngeal swab specimens. In addition, no viable virus could be cultured from swab specimens collected from the late-phase COVID-19 patients with prolonged viral RNA shedding.
CONCLUSIONS: In conclusion, our study suggests that even if viral shedding is sustained in asymptomatic or mildly symptomatic patients with later phase of COVID-19, it can be expected that the transmission risk of the virus is low. In addition, saliva can be used as a reliable specimen for the diagnosis of SARS-CoV-2 infection.
keyword
COVID-19; RT-PCR; coronavirus disease 2019; saliva; viral culture
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