Sharma, Prashant; Shin, Ji Beom; Park, Bum Chul; Lee, Jae-Won; Byun, Sang Won; Jang, Na-Yoon; Kim, Yu Jin; Kim, Yuri; Kim, Young Keun; Cho, Nam-Hyuk
Nanoscale
2019Feb ; 24 ( 5 ) :.
PMID : 30809611
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Sharma, Prashant - Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea and Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea. chonh@snu.ac.kr.
Shin, Ji Beom - Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea. ykim97@korea.ac.kr.
Park, Bum Chul - Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea. ykim97@korea.ac.kr.
Lee, Jae-Won - Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea and Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea. chonh@snu.ac.kr.
Byun, Sang Won - Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea. ykim97@korea.ac.kr.
Jang, Na-Yoon - Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea and Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea. chonh@snu.ac.kr.
Kim, Yu Jin - Research Center for Biomedical Nanocrystals, Korea University, Seoul 02841, Republic of Korea.
Kim, Yuri - Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea and Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea. chonh@snu.ac.kr.
Kim, Young Keun - Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea. ykim97@korea.ac.kr and Research Center for Biomedical Nanocrystals, Korea University, Seoul 02841, Republic of Korea.
Cho, Nam-Hyuk - Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea and Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea. chonh@snu.ac.kr and Institute of Endemic Disease, Seoul National University Medical Research Center and Bundang Hospital, Seoul 03080, Republic of Korea.
ABSTRACT
Zinc oxide (ZnO)-based nanocomposites have shown promising potential for various biomedical applications, including vaccine development, owing to their multifunctionality and biocompatibility. Here, we synthesized radially grown ZnO nanowires (NWs) on poly-l-lactic acid (PLLA) microfibers with unique 3-dimensional structure and applied them as therapeutic cancer vaccines. This inorganic-organic hybrid nanocomposite has mild cellular toxicity but efficiently delivers a tumor antigen into dendritic cells, cellular bridges between innate and adaptive immunity, to stimulate them to express inflammatory cytokines and activation surface markers. We also demonstrated that the hybrid nanocomposites successfully induce tumor antigen-specific cellular immunity and significantly inhibit tumor growth in vivo. ZnO NWs on PLLA fibers systemically reduced immune suppressive TReg cells and enhanced the infiltration of T cells into tumor tissues, compared to mice immunized with PLLA fibers coated with the antigen. Our current findings open a new avenue in extending the biomedical application of inorganic metal oxide-inert organic hybrid nanocomposites as a novel vaccine platform.
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