Lee, Hyeongjin; Yang, Gi Hoon; Kim, Minseong; Lee, JaeYoon; Huh, JunTae; Kim, GeunHyung
Materials science & engineering. C, Materials for biological applications
2018Mar ; 84 ( 2 ) :140-147.
PMID : 29519423
ÀúÀÚ »ó¼¼Á¤º¸
Lee, Hyeongjin - Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea.
Yang, Gi Hoon - Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea.
Kim, Minseong - Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea.
Lee, JaeYoon - Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea.
Huh, JunTae - Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea.
Kim, GeunHyung - Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon 440-746, Republic of Korea. Electronic address gkimbme@skku.edu.
ABSTRACT
Biomaterials must be biocompatible, biodegradable, and mechanically stable to be used for tissue engineering applications. Among various biomaterials, a natural-based biopolymer, collagen, has been widely applied in tissue engineering because of its outstanding biocompatibility. However, due to its low mechanical properties, collagen has been a challenge to build a desired/complex 3D porous structure with appropriate mechanical strength. To overcome this problem, in this study, we used a low temperature printing process to create a 3D porous scaffold consisting of collagen, decellularized extracellular matrix (dECM) to induce high cellular activities, and silk-fibroin (SF) to attain the proper mechanical strength. To show the feasibility of the scaffold, pre-osteoblast (MC3T3-E1) cells were grown on the fabricated scaffold. Various in vitro cellular activities (cell-viability, MTT assay, and osteogenic activity) for pure collagen, collagen/dECM, and collagen/SF/dECM scaffolds were compared. CI - Copyright ??2017 Elsevier B.V. All rights reserved.
na
¸µÅ©