Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species.

Lee, Mi Jin; Lee, Seung Jun; Yun, Su Jin; Jang, Ji-Young; Kang, Hangoo; Kim, Kyongmin; Choi, In-Hong; Park, Sun
International journal of nanomedicine
2016NA ; 11 ( 1 ) :55-68.
저자 상세정보
Lee, Mi Jin -
Lee, Seung Jun -
Yun, Su Jin -
Jang, Ji-Young -
Kang, Hangoo -
Kim, Kyongmin -
Choi, In-Hong -
Park, Sun -
ABSTRACT
The silver nanoparticle (AgNP) is a candidate for anticancer therapy because of its effects on cell survival and signaling. Although numerous reports are available regarding their effect on cell death, the effect of AgNPs on metabolism is not well understood. In this study, we investigated the effect of AgNPs on glucose metabolism in hepatoma cell lines. Lactate release from both HepG2 and Huh7 cells was reduced with 5 nm AgNPs as early as 1 hour after treatment, when cell death did not occur. Treatment with 5 nm AgNPs decreased glucose consumption in HepG2 cells but not in Huh7 cells. Treatment with 5 nm AgNPs reduced nuclear factor erythroid 2-like 2 expression in both cell types without affecting its activation at the early time points after AgNPs' treatment. Increased reactive oxygen species (ROS) production was detected 1 hour after 5 nm AgNPs' treatment, and lactate release was restored in the presence of an ROS scavenger. Our results suggest that 5 nm AgNPs affect glucose metabolism by producing ROS.
keyword
carbohydrate metabolism; cytotoxicity; lactic acid; metal nanoparticles
링크

주제코드
주제명(Target field)
연구대상(Population)
연구참여(Sample size)
대상성별(Gender)
질병특성(Condition Category)
연구환경(Setting)
연구설계(Study Design)
연구기간(Period)
중재방법(Intervention Type)
중재명칭(Intervention Name)
키워드(Keyword)
유효성결과(Recomendation)
5 nm AgNPs affect glucose metabolism by producing reactive oxygen species (ROS); This study demonstrated ROS-dependent reduction of lactate release by hepatoma cell lines in response to 5 nm AgNPs.
연구비지원(Fund Source)
근거수준평가(Evidence Hierarchy)
출판년도(Year)
참여저자수(Authors)
대표저자
DOI
10.2147/IJN.S94907
KCD코드
ICD 03
건강보험코드