Shim, Jin-Hyung; Yoon, Min-Chul; Jeong, Chang-Mo; Jang, Jinah; Jeong, Sung-In; Cho, Dong-Woo; Huh, Jung-Bo
Biomedical materials (Bristol, England)
2014Dec ; 9 ( 6 ) :065006.
PMID : 25384105
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Shim, Jin-Hyung -
Yoon, Min-Chul -
Jeong, Chang-Mo -
Jang, Jinah -
Jeong, Sung-In -
Cho, Dong-Woo -
Huh, Jung-Bo -
ABSTRACT
We successfully fabricated a three-dimensional (3D) printing-based PCL/PLGA/β-TCP guided bone regeneration (GBR) membrane that slowly released rhBMP-2. To impregnate the GBR membrane with intact rhBMP-2, collagen solution encapsulating rhBMP-2 (5 μg ml(-1)) was infused into pores of a PCL/PLGA/β-TCP membrane constructed using a 3D printing system with four dispensing heads. In a release profile test, sustained release of rhBMP-2 was observed for up to 28 d. To investigate the efficacy of the GBR membrane on bone regeneration, PCL/PLGA/β-TCP membranes with or without rhBMP-2 were implanted in an 8 mm calvaria defect of rabbits. Bone formation was evaluated at weeks 4 and 8 histologically and histomorphometrically. A space making ability of the GBR membrane was successfully maintained in both groups, and significantly more new bone was formed at post-implantation weeks 4 and 8 by rhBMP-2 loaded GBR membranes. Interestingly, implantation with rhBMP-2 loaded GBR membranes led to almost entire healing of calvaria defects within 8 weeks.
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MESH
Animals, Biocompatible Materials, Bone Morphogenetic Protein 2/*chemistry, *Bone Regeneration, Bone and Bones/*pathology, Calcium Phosphates/*chemistry, Collagen/chemistry, Lactic Acid/*chemistry, Membranes, Artificial, Microscopy, Electron, Scanning, Osteogenesis, Polyesters/*chemistry, Polyglycolic Acid/*chemistry, *Printing, Three-Dimensional, Rabbits, Recombinant Proteins/chemistry, Skull/*drug effects/pathology
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