Effects of the interleukin-1¥â-511 C/T gene polymorphism on the risk of gastric cancer in the context of the relationship between race and H. pylori infection: a meta-analysis of 20,000 subjects.

Park, Min-Jeong; Hyun, Myung-Han; Yang, Jong-Pill; Yoon, Jeong-Min; Park, Sungsoo
Molecular biology reports
2015Jan ; 42 ( 1 ) :119-34.
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Park, Min-Jeong -
Hyun, Myung-Han -
Yang, Jong-Pill -
Yoon, Jeong-Min -
Park, Sungsoo -
ABSTRACT
The interleukin (IL)-1β-511 C/T polymorphism has been shown to be functional and to contribute to the risk of gastric cancer. However, the relationship between the IL-1β-511 C/T polymorphism and gastric carcinogenesis remains inconclusive. A systematical electronic search was conducted of the MEDLINE, EMBASE, and CENTRAL databases. A random and a fixed effects model were exploited to estimate summary odds ratios and 95 % confidence intervals. Subgroup and sensitivity analyses were carried out with respect to ethnicity, quality assessment scores, control sources, genotyping methods, cancer histopathology and location, and Helicobacter pylori (H. pylori) infection. A total of 45 studies containing 9,066 cases of gastric cancer and 11,192 control subjects satisfied the inclusion criteria. The IL-1β-511 C/T polymorphism was found to enhance the risk of stomach cancer for overall and HWE-satisfying studies. Asians showed a positive relationship in both the overall and HWE-satisfying groups, whereas Caucasians did not. Based on subgroup analysis, H. pylori infection and genotype analysis using PCR-RFLP methods increase the association between IL-1β-511 T allele carrier and risk of stomach cancer. A positive relationship was found between the IL-1β-511 C/T SNP and stomach carcinoma susceptibility, and the results suggest that Asian ethnicity, H. pylori infection and methodologically, PCR-RFLP genotyping strengthen this relationship. Reflecting on prevalence of H. pylori in Asian countries, additional studies on the IL-1β-511 C/T SNP in the context of ethnicity and H. pylori infection may provide key insights into the mechanism underlying gastric cancer carcinogenesis. It was found PCR-RFLP is the most reliable genotyping method, and thus, it is recommendable to adopt it to determine the presence of the IL-1β-511 C/T SNP.
Interleukin-1beta, IL-1¥â-511 Cytokine Stomach neoplasm Single-nucleotide polymorphism
MESH
Continental Population Groups/*genetics, *Genetic Predisposition to Disease, Helicobacter Infections/*complications, Helicobacter pylori/physiology, Humans, Interleukin-1beta/*genetics, Linkage Disequilibrium/genetics, Polymorphism, Single Nucleotide/*genetics, Publication Bias, Risk Factors, Stomach Neoplasms/ethnology/*genetics/*microbiology
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The most reliable genotyping technique appears to be PCR-RFLP, which suggests that it should be used to analyze the relationship between the IL-1¥â-511 C/T SNP and the risk of stomach carcinoma.
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DOI
10.1007/s11033-014-3748-7.
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ICD 03
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