Chang, Ji-Eun; Yoon, In-Soo; Sun, Ping-Li; Yi, Eunjue; Jheon, Sanghoon; Shim, Chang-Koo
Journal of photochemistry and photobiology. B, Biology
2014Nov ; 140 ( 11 ) :49-56.
PMID : 25090224
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Chang, Ji-Eun -
Yoon, In-Soo -
Sun, Ping-Li -
Yi, Eunjue -
Jheon, Sanghoon -
Shim, Chang-Koo -
ABSTRACT
Photodynamic therapy (PDT) in combination with chemotherapy has great potential for cancer treatment. However, there have been very few attempts to developing cancer-targeted co-delivered systems of photosensitizers and anticancer drugs. We developed hematoporphyrin (HP)-modified doxorubicin (DOX)-loaded nanoparticles (HP-NPs) to improve the therapeutic effect of PDT in treating liver cancer. HP is not only a ligand for low density lipoprotein (LDL) receptors on the hepatoma cells but also a well-known photosensitizer for PDT. In vitro phototoxicity in HepG2 (human hepatocellular carcinoma) cells and in vivo anticancer efficacy in HepG2 tumor-bearing mice of free HP and HP-NPs were evaluated. The in vitro phototoxicity in HepG2 cells determined by MTT assay, annexin V-FITC staining and FACS analysis was enhanced in HP-NPs compared with free HP. Furthermore, compared with free HP-based PDT, in vivo anticancer efficacy in HepG2 tumor-bearing mice was markedly improved by HP-NPs-based PDT. Moreover, in both cases, the therapeutic effect was increased according to the irradiation time and number of PDT sessions. In conclusion, the HP-NPs prepared in this study represent a potentially effective co-delivery system of photosensitizer (HP) and anticancer drug (DOX) which improved the effects of PDT in liver cancer. CI - Copyright ??2014 Elsevier B.V. All rights reserved.
keyword
Doxorubicin; Hematoporphyrin; Liver cancer; Nanoparticles; Photodynamic therapy
MESH
Animals, Antineoplastic Agents/administration & dosage/*chemistry/pharmacology, Apoptosis/drug effects/radiation effects, Carcinoma, Hepatocellular/drug therapy, Doxorubicin/administration & dosage/*chemistry/pharmacology, Drug Carriers/chemistry, Hematoporphyrins/*chemistry/pharmacology, Hep G2 Cells, Humans, Light, Liver Neoplasms/drug therapy, Mice, Mice, Inbred BALB C, Mice, Nude, Nanoparticles/*chemistry, Photochemotherapy, Photosensitizing Agents/*chemistry/pharmacology, Transplantation, Heterologous
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