Lee, Gyu-Cheol; Jeon, Eun-Sung; Le, Dung Tien; Kim, Tong-Soo; Yoo, Jong-Ha; Kim, Hak Yong; Chong, Chom-Kyu
The American journal of tropical medicine and hygiene
2011Dec ; 85 ( 6 ) :989-93.
PMID : 22144432
저자 상세정보
Lee, Gyu-Cheol -
Jeon, Eun-Sung -
Le, Dung Tien -
Kim, Tong-Soo -
Yoo, Jong-Ha -
Kim, Hak Yong -
Chong, Chom-Kyu -
ABSTRACT
Plasmodium falciparum and P. vivax malaria are endemic to many parts of the world and humans can be co-infected with both species. Because each Plasmodium species has different biological and clinical characteristics, accurate differentiation of the infecting species is essential for effective treatment. Therefore, we produced three monoclonal antibodies that recognize the lactate dehydrogenase of P. falciparum, P. vivax, or both to develop the first P. falciparum, P. vivax, and mixed-species infections malaria antigen detection kit. The detection limits of this kit were 150 and 250 parasites/關L for P. falciparum and P. vivax, respectively, and the kit was able to detect mixed-species infections. The sensitivity and specificity of this kit was assessed with 722 clinical specimens. Our results showed that its sensitivities for P. falciparum, P. vivax, and mixed-species infection were 96.5%, 95.3%, and 85.7%, respectively. In addition, its specificity was high (99.4%).
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MESH
Antibodies, Monoclonal/immunology, Antigens, Protozoan/*immunology, Humans, L-Lactate Dehydrogenase/immunology, Malaria, Falciparum/*diagnosis/immunology, Malaria, Vivax/*diagnosis/immunology, Plasmodium falciparum/*immunology, Plasmodium vivax/*immunology, Reagent Kits, Diagnostic/parasitology, Sensitivity and Specificity
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