±èÁ¤¹Î(Kim Jeong-Min) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
Á¤À±¼®(Chung Yoon-Seok) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
Á¶ÇýÁØ(Jo Hye-Jun) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
À̳²ÁÖ(Lee Nam-Joo) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
±è¹Ì¼±(Kim Mi-Seon) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
¿ì»óÈñ(Woo Sang-Hee) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
¹Ú¼¼Èñ(Park Se-Hee) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
±èÁö¿õ(Kim Jee-Woong) - Korea Centers for Disease Control and Prevention National Institute of Health Division of Biosafety Evaluation and Control
±èÈñ¸¸(Kim Heui-Man) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
ÇÑ¸í±¹(Han Myung-Guk) - Korea Centers for Disease Control and Prevention Center for Laboratory Control of Infectious Diseases Division of Viral Diseases
Abstract
Objectives: Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
Methods: Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
Results: The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
Conclusion: SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
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COVID-19, coronavirus, SARS-CoV-2, isolation, pneumonia
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SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.