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Anti-cancer Effects of Cultivated Orostachys japonicus on Human Colon Cancer Cell Line SW480
Journal of Life Science 2018³â 28±Ç 7È£ p.819 ~ p.826
¹Ú¼ö°æ(Park Soo-Kyoung) - ÀÎÁ¦´ëÇб³ º¸°ÇÀÇ·áÀ¶ÇÕ´ëÇÐ ¹°¸®Ä¡·áÇаú
¿øÁø¿µ(Won Jin-Young) - ÀÎÁ¦´ëÇб³ ´ëÇпø ÀçÈ°°úÇаú
¹Ú°Èñ(Park Kang-Hui) - µ¿ÁÖ´ëÇб³ ¹°¸®Ä¡·á°ú
È«¿ë±Ù(Hong Yong-Geun) - ÀÎÁ¦´ëÇб³ ´ëÇпø ÀçÈ°°úÇаú
Abstract
¿Í¼Û(µÕ±Ù¹ÙÀ§¼Ö, Orostachys japonicus, OJ)Àº ¸é¿ªÁ¶Àý, Ç׳ëÈ, Ç×»êÈ, µ¶¼ºÁ¦°Å µîÀÇ Ä¡·áÀû È¿°ú¸¦ °¡Áø ¾à¿ë½Ä¹°·Î ¾Ë·ÁÁ® Àִµ¥, º» ¿¬±¸¿¡¼´Â ÀÎü´ëÀå¾Ï¼¼Æ÷¿¡ ´ëÇÑ Àç¹è ¿Í¼ÛÀÇ Ç×¾ÏÈ¿°ú¸¦ ±Ô¸íÇÏ°íÀÚ ÇÏ¿´´Ù. ÀÎü´ëÀå¾Ï¼¼Æ÷ÁÖ SW480¿¡ ¿Í¼Û¿¼öÃßÃâ¹°À» 72½Ã°£ µ¿¾È ó¸®ÇÑ °á°ú, ´ëÀå¾Ï¼¼Æ÷ÀÇ »ýÁ¸À²Àº ³óµµ-ÀÇÁ¸ÀûÀ¸·Î À¯ÀÇÇÏ°Ô °¨¼ÒÇÏ¿´´Ù. ¶ÇÇÑ, ¿Í¼ÛÀº SW480 ¼¼Æ÷ÀÇ Áõ½Ä°ú ÀÌÁÖ¸¦ ¾ïÁ¦ÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Âµ¥, ´ëÁ¶±ºÀÇ ½ºÅ©·¡Ä¡ gapÀº 24½Ã°£ºÎÅÍ À¯ÀÇÇÏ°Ô °¨¼ÒÇÏ´Â ¹Ý¸é, ¿Í¼Û¿¼öÃßÃâ¹°À» ó¸®ÇÑ ½ÇÇ豺 I°ú IIÀÇ ½ºÅ©·¡Ä¡ gapÀº 48½Ã°£ºÎÅÍ °¨¼ÒÇϱ⠽ÃÀÛÇÏ¿´´Ù. ƯÈ÷ ½ÇÇ豺 IÀÇ ½ºÅ©·¡Ä¡ gapÀº 72½Ã°£±îÁö À¯ÀÇÇÏ°Ô À¯ÁöµÇ¾ú´Ù. ¿Í¼ÛÀÌ »ýü ³»¿¡¼ Á¾¾çÀÇ Çü¼º¿¡ ¾î¶°ÇÑ ¿µÇâÀ» ¹ÌÄ¡´ÂÁö ¾Ë¾Æº¸±â À§ÇÏ¿© ´ëÀå¾Ï¼¼Æ÷ ÁÖ»ç Àü, 31ÀÏ µ¿¾È ¼öÄÆ C57BL/6 ¸¶¿ì½º(4ÁÖ·É)¿¡°Ô ¿Í¼Û¿¼öÃßÃâ¹°À» °æ±¸·Î ÀÚÀ¯ ¼·ÃëÇÏ°Ô ÇÏ¿´´Ù. ÀÌÈÄ SW480 ¼¼Æ÷(1¡¿107 cells/100 ¥ìl)¸¦ ÇÇÇÏ·Î ÁÖÀÔÇÑ ÈÄ 7ÀÏ, 14ÀÏ ±×¸®°í 28ÀÏ¿¡¼ Á¾¾çÀÇ Çü¼ºÀ» °üÂûÇÏ¿´°í, °¢ ½ÇÇ赿¹°À» Èñ»ýÇÏ¿© Á¾¾çÀÇ ¹«°Ô¿Í ºÎÇÇ(mm3)¸¦ ÃøÁ¤ÇÏ¿© ºñ±³ ºÐ¼®ÇÏ¿´´Ù. ¿Í¼Û¿¼öÃßÃâ¹°À» ¼·ÃëÇÏ´Â µ¿¾È ½ÇÇ豺 I, IIÀÇ Ã¼ÁßÀº ´ëÁ¶±º¿¡ ºñÇØ Áö¼ÓÀûÀ¸·Î À¯ÀÇÇÏ°Ô Áõ°¡ÇÏ¿´´Ù. SW480 ¼¼Æ÷ ÁÖÀÔ ÀÌÈÄ ¸ðµç ½ÇÇ豺ÀÇ Ã¼ÁßÀº °¨¼ÒÇÏ¿´À¸³ª, ¼¼Æ÷ ÁÖÀÔ ÈÄ 14ÀϺÎÅÍ ¿Í¼Û ¼·Ãë ½ÇÇ豺ÀÇ Ã¼ÁßÀº À¯ÀÇÇÏ°Ô Áõ°¡ÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ´ëÀå¾Ï¼¼Æ÷ ÁÖÀÔ ÈÄ 7ÀÏ°ú 14ÀÏ¿¡¼ ¿Í¼ÛÀ» ¼·ÃëÇÑ ½ÇÇ豺ÀÇ Á¾¾ç ¹«°Ô¿Í ºÎÇÇ´Â ´ëÁ¶±ºº¸´Ù ³ô¾ÒÀ¸³ª, 28ÀÏ¿¡¼´Â ´ëÁ¶±ºº¸´Ù ³·°Ô ³ªÅ¸³µ°í, ƯÈ÷ ½ÇÇ豺 II¿¡¼ Á¾¾ç ¹«°Ô¿Í ºÎÇÇ´Â À¯ÀÇÇÏ°Ô °¨¼ÒÇÏ´Â °ÍÀ¸·Î È®ÀεǾú´Ù. ÀÌ»óÀÇ °á°ú¸¦ ÅëÇØ ¿Í¼ÛÀÌ ÀÎü ´ëÀå¾Ï¼¼Æ÷ÀÇ ¼ºÀå, Áõ½Ä ¹× ÀÌÁÖ¸¦ ¾ïÁ¦Çϸç, »ýü ³» Á¾¾çÇü¼º(tumorigenesis)À» ¿¹¹æÀûÀ¸·Î ¾ïÁ¦ÇÔÀ» ¾Ë ¼ö ÀÖ¾ú´Ù. µû¶ó¼, Àç¹è ¿Í¼ÛÀº õ¿¬ Ç×¾ÏÁ¦ ¼ÒÀç·Î¼ È°¿ëµÉ °¡´É¼ºÀ» °¡Áö´Â °ÍÀ¸·Î º¸À̸ç, ÀÌ¿¡ ´ëÇÑ ½ÉÃþÀûÀÎ ¿¬±¸°¡ ÇÊ¿äÇÒ °ÍÀ¸·Î »ç·áµÈ´Ù.
Orostachys japonicus (OJ) is a medicinal herb with immunoregulatory, anti-aging, anti-oxidative, and many other therapeutic properties. The purpose of this study was to elucidate the anti-cancer property of cultivated OJ. SW480 cell viability was significantly reduced by cumulative exposure to OJ extract. We also observed inhibitory effects of OJ after 72 hr through the growth and migration of SW480 cells using scratch assay. SW480 cells in OJ-free medium began to move into the scratch site at 24 hr; however, cells in medium containing OJ did not migrate into the scratch site until 48 hr. Male C57BL/6 mice (4 weeks old) were orally administered OJ extract for 31 days before injection of SW480 cells. At 7, 14, and 28 days after subcutaneous injection of SW480 cells, tumor weight and volume were analyzed. The body weight of the OJ-treated group was continuously increased during administration of the OJ extract relative to the control group. Injection of SW480 cells caused a reduction in body weight in all groups; however, the OJ-treated group exhibited a significant increase in body weight after 14 days. Tumor weight and volume were lower in the OJ-treated group than in the control group after 28 days. Although these results suggest that OJ suppresses the proliferation and migration of human colon cancer cells, additional studies are required to provide preclinical evidence before launching clinical trials evaluating OJ as an anti-cancer biohealth product.
Å°¿öµå
Anti-cancer effect, cancer cell migration, human colon cancer cells, Orostachys japonicus
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À¯È¿¼º°á°ú(Recomendation)
½ÇÇ豺 II¿¡¼ Á¾¾ç ¹«°Ô¿Í ºÎÇÇ´Â À¯ÀÇÇÏ°Ô °¨¼ÒÇÏ´Â °ÍÀ¸·Î È®ÀÎ; ¿¬±¸°á°ú¸¦ ÅëÇØ ¿Í¼ÛÀÌ ÀÎü ´ëÀå¾Ï¼¼Æ÷ÀÇ ¼ºÀå, Áõ½Ä ¹× ÀÌÁÖ¸¦ ¾ïÁ¦Çϸç, »ýü ³» Á¾¾çÇü¼º(tumorigenesis)À» ¿¹¹æÀûÀ¸·Î ¾ïÁ¦ÇÔ