Alternative Carcinogenicity Screening Assay Using Colon Cancer Stem Cells: A Quantitative PCR (qPCR)-Based Prediction System for Colon Carcinogenesis

Journal of Microbiology and Biotechnology 2018년 28권 4호 p.645 ~ p.651

(Bak Ye-Sol) - Konkuk University Department of Bioscience and Biotechnology
(Jang Hui-Joo) - Konkuk University Department of Bioscience and Biotechnology
(Shin Jong-Woon) - Konkuk University Department of Bioscience and Biotechnology
김수진(Kim Soo-Jin) - Konkuk University Department of Bioscience and Biotechnology
(Chun Hyun-Woo) - Konkuk University Department of Bioscience and Biotechnology
서지혜(Seo Ji-Hye) - Jeonbuk National University School of Dentistry Department of Dental Pharmacology
(No Su-Hyun) - Jeonbuk National University School of Dentistry Department of Dental Pharmacology
채정일(Chae Jung-Il) - Jeonbuk National University School of Dentistry Department of Dental Pharmacology
손동희(Son Dong-Hee) - Sejong University College of Natural Sciences Department of Applied Statistics
이승연(Lee Seung-Yeoun) - Sejong University College of Natural Sciences Department of Applied Statistics
홍진태(Hong Jin-Tae) - Chungbuk National University College of Pharmacy
윤도영(Yoon Do-Young) - Konkuk University Department of Bioscience and Biotechnology

Abstract

The carcinogenicity of chemicals in the environment is a major concern. Recently, numerous studies have attempted to develop methods for predicting carcinogenicity, including rodent and cell-based approaches. However, rodent carcinogenicity tests for evaluating the carcinogenic potential of a chemical to humans are time-consuming and costly. This study focused on the development of an alternative method for predicting carcinogenicity using quantitative PCR (qPCR) and colon cancer stem cells. A toxicogenomic method, mRNA profiling, is useful for predicting carcinogenicity. Using microarray analysis, we optimized 16 predictive gene sets from five carcinogens (azoxymethane, 3,2’-dimethyl-4-aminobiphenyl, N-ethyl-n-nitrosourea, metronidazole, 4-(n-methyl-n-nitrosamino)-1-(3-pyridyl)-1-butanone) used to treat colon cancer stem cell samples. The 16 genes were evaluated by qPCR using 23 positive and negative carcinogens in colon cancer stem cells. Among them, six genes could differentiate between positive and negative carcinogens with a p-value of ≤0.05. Our qPCR-based prediction system for colon carcinogenesis using colon cancer stem cells is cost- and time-efficient. Thus, this qPCR-based prediction system is an alternative to in vivo carcinogenicity screening assays.

키워드

Microarray, quantitative PCR, colon cancer, cancer stem cell, carcinogenicity
원문 및 링크아웃 정보
등재저널 정보
SCI(E) MEDLINE 학술진흥재단(KCI) 
주제코드
주제명(Target field)
연구대상(Population)
연구참여(Sample size)
대상성별(Gender)
질병특성(Condition Category)
연구환경(Setting)
연구설계(Study Design)
연구기간(Period)
중재방법(Intervention Type)
중재명칭(Intervention Name)
키워드(Keyword)
유효성결과(Recomendation)
This qPCR-based prediction system is an alternative to in vivo carcinogenicity screening assays.
연구비지원(Fund Source)
근거수준평가(Evidence Hierarchy)
출판년도(Year)
참여저자수(Authors)
대표저자
KCD코드
ICD 03
건강보험코드