Virus Isolation from the First Patient with SARS-CoV-2 in Korea

Journal of Korean Medical Science 2020³â 35±Ç 7È£ p.84 ~ p.84

¹Ú¿Ï¹ü(Park Wan-Beom) - Seoul National University College of Medicine Department of Internal Medicine
(Kwon Nak-Jung) - Macrogen
ÃÖ¼öÁø(Choi Su-Jin) - Seoul National University Hospital Biomedical Research Institute Laboratory of Infection and Immunity
°­Ã¢°æ(Kang Chang-Kyung) - Seoul National University College of Medicine Department of Internal Medicine
ÃÖÆò±Õ(Choe Pyoeng-Gyun) - Seoul National University College of Medicine Department of Internal Medicine
±èÁø¿ë(Kim Jin-Yong) - Incheon Medical Center Department of Internal Medicine
À±Áö¿µ(Yun Ji-Young) - Macrogen
(Lee Gir-Won) - Macrogen
¼º¹®¿ì(Seong Moon-Woo) - Seoul National University College of Medicine Department of Laboratory Medicine
±è³²Áß(Kim Nam-Joong) - Seoul National University College of Medicine Department of Internal Medicine
¼­Á¤¼±(Seo Jeong-Sun) - Macrogen
¿À¸íµ·(Oh Myoung-Don) - Seoul National University College of Medicine Department of Internal Medicine

Abstract

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

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2019 Novel Coronavirus, Culture, Microscopy, Electron, Phylogeny, Korea
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Phylogenetic analyses of the whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan, China.
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